Document 0799
 DOCN  M94B0799
 TI    Edema induced by factors released by AIDS-associated Kaposi's sarcoma
       cells (AIDS-KS cell) (Meeting abstract).
 DT    9412
 AU    Nakamura S; Murakami-Mori K; Sakurada S; Rajeeve S; Rao N; Weich H;
       Salahuddin SZ; Inst. of Molecular Medicine and Technology, Pasadena, CA
       91105
 SO    Proc Annu Meet Am Assoc Cancer Res; 35:A402 1994. Unique Identifier :
       AIDSLINE ICDB/94602090
 AB    Angiogenesis and in some cases edema are two major components of
       AIDS-KS. Cultured spindle cells isolated from various KS lesions of
       patients with AIDS transplanted in nude mice induce KS-like lesions of
       murine origin. The critical factor for this purpose is oncostatin H. We
       report here that AIDS-KS cells also release another factor that mediates
       vascular hyperpermeability (VHP) resulting in edema in our model system.
       This factor in CH induces VHP and growth of vascular endothelial cells.
       These activities bind heparin sepharose and elute in 0.5 M NaCl
       fraction. The endothelial cell growth promoting activities were
       identified in 0.5 M and 2.0 M NaCl fractions. Anti-bFGF IgG inhibited
       endothelial cell growth promoting activity in 2.0 M NaCl fraction.
       Anti-VEGF serum inhibited VHP and endothelial cell growth promoting
       activities of 0.5 M NaCl fraction. Both 0.5 M and 2.0 M NaCl fractions
       induced angiogenesis in rabbit cornea assay. Significant level of
       VEGF-specific mRNA was present in AIDS-KS cells (3.9 kb band). In
       summary, it appears that AIDS-KS cells produce VEGF and bFGF-like
       activities that mediate two important features of AIDS-KS: edema and
       angiogenesis.
 DE    Acquired Immunodeficiency Syndrome/COMPLICATIONS/*PATHOLOGY  Animal
       Cell Division  Edema/*ETIOLOGY/METABOLISM  Endothelial Growth
       Factors/GENETICS/IMMUNOLOGY  Endothelium, Vascular/*PATHOLOGY
       Fibroblast Growth Factor, Basic/IMMUNOLOGY  Human  IgG/PHARMACOLOGY
       Lymphokines/GENETICS/IMMUNOLOGY  Mice  Mice, Nude
       Neovascularization/*METABOLISM/PATHOLOGY  RNA, Messenger/METABOLISM
       Sarcoma, Kaposi's/ETIOLOGY/*PATHOLOGY/SECRETION  MEETING ABSTRACT

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